Dengue hemmorrhagic fever (DHF) is the one of vector born diseases which is a one a public health problem in Indonesia. The distribution and number of DHF cases are increases in Indonesia every years. The vector control using larvicides should be tested to know the effectivity. This reports describes the efficacy of larvacide altosid® 1.3 g for DHF vector Aedes aegypti control in three doses 2.5%, 4% and 5%. This study was carried in laboratorium of Health Ecology Research Centre in 2010 by using WHO methods. The results shows the lethal concentration (LC50) of altosid was 2.12%, and LC 95 was 5.52%. The lethal time (LT 50) of altosid at 2.5% concentration was 8.02% and LT 95 was 12.50 %. The LT 50 and LT 95 of altosid 4% were 6.52 and 12.50 consequently, and LT 50 and LT 95 at concentration 5% were 6.72 and 9.64 consequently. The concentration of altosid at 2.5 % larva mortality was 6.4%, though at concentration 4 %, 84.8 % larvae already become pupae at the eight days after treatment, nevertheless, only 11.2% become adults stage. At the concentration of 5% have 72% pupa is formed, 10.4% were able to continue its growth into a mosquito, but eventually experience death due to abnormal growth, while the pupa is in its development has seen an abnormal experience death before they become mosquitoes. The abnormality of pupa case by this larvicide function as mimic hormon which has a mechanism to regulate the development of larva stage (IGR-Insect Growth Regulator). The mode of action of this hormon is not directly killing the larva, but to hamper the chytine formation, at the end pupa will not able to become adults or the adults stage will be abnormal.