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Chapter 7 - Combining Wide-Field Super-Resolution Microscopy and Electron Tomography: Rendering Nanoscopic Correlative Arrays on Subcellular Architecture

Elsevier Science & Technology
DOI: 10.1016/b978-0-12-801075-4.00007-0
  • Actin
  • Colon Cancer
  • Correlating Single Molecules
  • Correlative Morphomics
  • Correlative Pointillism
  • Correlative Nanoscopy
  • Cytoskeleton
  • Ground State Depletion
  • Fine Structure Immunocytochemistry
  • Fluorescent Probes
  • Nanoscopy
  • Simulation
  • Three-Dimensional Modeling
  • Biology
  • Medicine


Abstract In this chapter, the authors outline in full detail, an uncomplicated approach that enables the combination of wide-field fluorescence super-resolution microscopy with electron tomography, thereby providing an approach that affords the best possible confidence in the structures investigated. The methodical steps to obtain these high-throughput correlative nanoscopic arrays will be visually explored and outlined in detail. The authors will demonstrate the feasibility of the method on cultured Caco-2 colorectal cancer cells that are labeled for filamentous actin. The presented images, morphometric data, and generated models illustrate the strengths of our correlative approach for future advanced structural-biology-oriented questions. Correlative nanoscopy applications can be readily found in which there is a need to reveal biomolecular information at unprecedented resolution on subcellular behavior in various biological and pathobiological processes.

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