Affordable Access

Publisher Website

Pepsins—A chromatographic and kinetic comparison

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
31
Identifiers
DOI: 10.1016/0003-2697(69)90252-8

Abstract

Abstract A simplified procedure for the preparation of pepsin from porcine pepsinogen has been developed. Chromatographic and kinetic data indicate that this procedure yields a pepsin product identical with that obtained by the conventional method. The K m and k cat values for the hydrolysis of Ac-Phe-Tyr-OMe by commercial and freshly prepared pepsins were determined. All pepsins studied were found to have the same value for k cat . The parameter which kinetically distinguished each of the pepsins was K m .

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Pepsins--a chromatographic and kinetic comparison.

on Analytical Biochemistry Oct 01, 1969

Kinetic comparison of human, canine and porcine pe...

on Comparative biochemistry and p... Aug 15, 1971

[Changes in the kinetics of heat inactivation of p...

on Casopís lékar̆ů c̆eských Jun 22, 1962
More articles like this..