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Chapter 8 Ultrafast Fluorescence Spectroscopy via Upconversion:Applications to Biophysics

Elsevier Science & Technology
DOI: 10.1016/s0076-6879(08)03408-3
  • Biology
  • Physics


Abstract This chapter reviews basic concepts of nonlinear fluorescence upconversion, a technique whose temporal resolution is essentially limited only by the pulse width of the ultrafast laser. Design aspects for upconversion spectrophotofluorometers are discussed, and a recently developed system is described. We discuss applications in biophysics, particularly the measurement of time‐resolved fluorescence spectra of proteins (with subpicosecond time resolution). Application of this technique to biophysical problems such as dynamics of tryptophan, peptides, proteins, and nucleic acids is reviewed.

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