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Studies on biotransformation of elastase. I. Transport of131I-labeled elastase across rat intestinein vitro

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
288
Issue
1
Identifiers
DOI: 10.1016/0005-2736(72)90235-0
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Elastase, an elastolytic enzyme isolated from hog pancrease, was labeled with 131I and its transport investigated using rat everted jejunum. The everted sac of rat jejunum was incubated in Krebs-Ringer bicarbonate solution containing 0.01 or 0.1% of 131I-labeled elastase. The fact that 131I-bound protein was transported from the mucosal to serosal side was confirmed by several methods such as precipitation by trichloroacetic acid, gel filtration and electrophoresis. It was also found that the transfer rate of 131I-bound protein agreed approximately with that determiend by the enzymatic and immunological methods.

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