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Experimental Pyelonephritis VI. Observations on Susceptibility of the Rabbit Kidney to Infection by a Virulent Strain of Staphylococcus Aureus **

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LAWRENCE R. FREEDMAN Department of Internal Medicine, YaleUniversity School of Medicine EXPERIMENTAL PYELONEPHRITIS VI. OBSERVATIONS ON SUSCEPTIBILITY OF THE RABBIT KIDNEY TO INFECTION BY A VIRULENT STRAIN OF STAPHYLOCOCCUS AUREUS** For many years it has been known that the intravenous injection of large numbers of virulent staphylococci into normal mice or rabbits regularly results in abscess formation in the kidneys."'7 These infections occur also in the rat.' Gram-negative bacilli of the coliform group, on the other hand, very rarely produce such infections when similarly administered to normal animals ; some form of renal injury is usually required for the establish- ment of experimental pyelonephritis by that group of bacteria. It seemed likely, therefore, that there might be significant differences in the pyelo- nephritis caused by these two kinds of microorganisms. The present article is a report of experiments designed to compare the course of staphylococcal infection in the rabbit kidney with that previously noted in experiments with coliform bacterial infections. MATERIALS AND METHODS Except where specified, the materials and methods were the same as those described in preceding reports from this laboratory."9"2 All experiments were carried out in white New Zealand male rabbits weighing between 2 and 3 kilograms. Staphylococcus aureus. This organism, known as Giorgio, was supplied to us by Dr. David Rogers of Vanderbilt Medical School. It had originally been isolated from a staphylococcal abscess in man. It is hemolytic and coagulase positive, and has been characterized in some detail by Smith and Dubos.'2 Intravenous injection of 1O organ- isms (18-hr. culture) into mice was found to cause the death of approximately two- thirds within 7 days. The organism was maintained at room temperature on agar slants. Tn the beginning of this study 6 agar slants were inoculated from a broth culture, and, after overnight incubation, were sealed with paraffin. A new slant was taken each month

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