Affordable Access

Publisher Website

Use of N-acetylpsychosine as internal standard for quantitative high-performance liquid chromatographic analysis of glycosphingolipids

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
555
Identifiers
DOI: 10.1016/s0021-9673(01)87188-0

Abstract

Abstract The use of N-acetylpsychosine as an internal standard forthe quantitative high-performance liquid chromatography (HPLC) of p-nitrobenzoyl derivatives of glycosphingolipids is described. It is suitable because the chromogen reacts on equimolar basis with both N-acetylpsychosine and sample glycosphingolipids. The use of N-acetylpsychosine as an internal standard was validated by determining the glycosphingolipid content of a system of metastatic variants selected from a murine fibrosarcoma line (T3 cells). Reproducible results were obtained throughout several quantitative analyses of cellular glycosphingolipids and it was possible to determine the glycosphingolipid content of as few as 5 · 10 6 cells.

There are no comments yet on this publication. Be the first to share your thoughts.