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Antisense HEMA1 RNA Expression Inhibits Heme and Chlorophyll Biosynthesis in Arabidopsis1

Authors
Publisher
American Society of Plant Physiologists
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

Antisense HEMA1 RNA Expression Inhibits Heme and Chlorophyll Biosynthesis in Arabidopsis by A. Madan Kumar, and Dieter Söll Plantphysiol Volume 122(1):49-56 January 1, 2000 ©2000 by American Society of Plant Biologists Schematic representation of the location ofHEMA1 (hma1) and HEMA2 (hma2) genes on the Arabidopsis chromosome I. The distance (in centiMorgans [cM]) and the markers nearest to these genes are shown. Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists Phenotypes of the selected 3-week-old transgenic Arabidopsis plants. Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists Chlorophyll (top), protoheme (middle), and ALA (bottom) levels in 2-week-old Arabidopsis transgenic lines. Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists DNA analysis of the transgenic Arabidopsis lines. Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists RNA analysis of the transgenic Arabidopsis lines expressing antisense HEMA1 RNA. Total RNA (20 μg) was isolated from 2-week-old Arabidopsis plants and blotted onto a nitrocellulose membrane as described in “Materials and Methods.” Lanes indicate the selecte... Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists Western-blot analysis of the transgenic Arabidopsis lines expressing antisense HEMA1 RNA. Lane 1, 2-1B2; lane 2, 2-10PG1; lane 3, 2-10PG2; lane 4, 17-10; and lane 5, control plant. Kumar A, Söll D. Plantphysiol 2000;122:49-56 ©2000 by American Society of Plant Biologists Schematic representation of the location ofHEMA1 (hma1) and HEMA2 (hma2) genes on the Arabidopsis chromosome I. The distance (in centiMorgans [cM]) and the markers nearest to these genes are shown. Numbers in parentheses indicate the marker identification. Phenotypes of the selected 3-week-old transgenic Arabidopsis plants. A control plant and four transgenic plants (2-1B2, 2

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