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Isolation of nuclear rapidly sedimenting ribonucleic acids by agarose gel filtration

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
49
Issue
2
Identifiers
DOI: 10.1016/0003-2697(72)90439-3

Abstract

Abstract Gel filtration of rat liver nuclear RNA's on Sepharose 4B permits routine separation of the rapidly sedimenting (30–60S) ribopolynucleotides from ribosomal RNA's. The method could be easily adapted to either analytical characterization or preparative isolation of preribosomal and polycistronic RNA's.

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