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3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

Authors
  • Meier, Sebastian
  • Benie, Andrew J
  • Duus, Jens Øllgaard
  • Sørensen, Ole W.
Publication Date
Jan 01, 2009
Source
Online Research Database In Technology
Keywords
License
Unknown
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Abstract

3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.

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