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3D differential phase-contrast microscopy with computational illumination using an LED array.

Authors
  • Tian, Lei
  • Wang, Jingyan
  • Waller, Laura
Type
Published Article
Journal
Optics Letters
Publisher
The Optical Society
Publication Date
Mar 01, 2014
Volume
39
Issue
5
Pages
1326–1329
Identifiers
DOI: 10.1364/OL.39.001326
PMID: 24690738
Source
Medline
License
Unknown

Abstract

We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational illumination with a programmable LED array. By capturing intensity images with various illumination angles generated by sequentially patterning an LED array source, we digitally refocus images through various depths via light field processing. The intensity differences from images taken at complementary illumination angles are then used to generate DPC images, which are related to the gradient of phase. The proposed method achieves 3D DPC with simple, inexpensive optics and no moving parts. We experimentally demonstrate our method by imaging a camel hair sample in 3D.

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