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Isolation of neurosecretory granules containing cytochromogenic hormone activity from the corpora cardiaca ofBlaberus discoidaliscockroaches

Authors
Journal
General and Comparative Endocrinology
0016-6480
Publisher
Elsevier
Publication Date
Volume
52
Issue
1
Identifiers
DOI: 10.1016/0016-6480(83)90168-5
Disciplines
  • Medicine

Abstract

Abstract Neurosecretory granules (NSG) were isolated by differential centrifugation from a homogenate of the corpora cardiaca-corpora allata complex of newly emerged, adult, female Blaberus discoidalis cockroaches. By centrifuging at 11,000 g, the procedure yielded a final pellet that was enriched in small, membrane-bound vesicles (103 nm mean diameter). This pellet contained 61% of the cytochromogenic activity present in the orginal homogenate at an 11-fold increase in concentration. A pellet formed at 4000 g contained a mixture of mitochondria and larger NSG (146 nm mean diameter) and most of the hindgut-stimulating hormone activity. This procedure provided a quick, simple method for purifying neurosecretory granules and separating them into two categories of size and endocrine content.

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