Affordable Access

Publisher Website

A simple and rapid method to determine the zygosity of uPA-transgenic SCID mice

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
308
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(03)01388-3
Keywords
  • Upa
  • Scid
  • Transplantation
  • Hepatocytes
  • Genotyping
  • Pcr
  • Animal Model
  • Hbv
  • Hcv
Disciplines
  • Medicine

Abstract

Abstract Successful transplantation of xenogeneic hepatocytes into uPA-transgenic SCID mice depends on the zygosity of the recipient mice. Normally, the difference between homozygous and heterozygous animals is determined via a quantitative Southern blot. We sequenced a part of the mouse genome that is eliminated upon integration of the transgene in the genome. Based on that sequence we developed a multiplex PCR that allows the unambiguous discrimination of negative, heterozygous, and homozygous uPA-transgenic SCID mice in a single day procedure. The speed of the procedure is an essential quality because transplantation of xenogeneic hepatocytes into uPA-SCID mice should be done as soon as possible after birth.

There are no comments yet on this publication. Be the first to share your thoughts.