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Characterizing the fitness cost of viral escape from the HIV-1 broadly neutralizing monoclonal antibody VRC01

Authors
Journal
Retrovirology
1742-4690
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
9
Identifiers
DOI: 10.1186/1742-4690-9-s2-p87
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

Characterizing the fitness cost of viral escape from the HIV-1 broadly neutralizing monoclonal antibody VRC01 POSTER PRESENTATION Open Access Characterizing the fitness cost of viral escape from the HIV-1 broadly neutralizing monoclonal antibody VRC01 RM Lynch1*, L Tran1, X Wu1, Y Li2, B Lee3, J Mascola1 From AIDS Vaccine 2012 Boston, MA, USA. 9-12 September 2012 Background The receptor-binding site on the HIV glycoprotein gp120 is a highly conserved epitope, and certain antibodies directed against this CD4 binding site (CD4bs) can potently neutralize the majority of circulating HIV-1 iso- lates. One such antibody, VRC01, was isolated from a slow progressor HIV-1 infected donor who maintained low to moderate viral load without treatment. We recently described that almost all viruses in this donor plasma had escaped VRC01 neutralization. This raised the question of whether viral escape from a broadly reac- tive CD4bs antibody results in reduced affinity for CD4 and thus, a fitness cost to viral replication. Methods Env-pseudoviruses and infectious molecular clones (IMC) were constructed using near-full length gp160 env genes from three circulating VRC01-resistant viruses and their complementary revertants (where VRC01-sensitivity was restored through mutations in the CD4 binding loop, Loop D and V5) as well as from autologous env genes from the VRC01 donor (both sensitive and resistant to VRC01 neutralization). Cell entry was quantified by infec- tivity into cell-lines expressing varying levels of the CD4 receptor, and replication kinetics of IMC were assessed by in vitro infection of primary CD4 T cells. Results Two of the three reverted VRC01-sensitive viruses demon- strated more efficient CD4 receptor mediated entry and greater replication in CD4 T cells, than the parental VRC01-resistant Envs. However, analysis of five VRC01- resistant and four VRC01-sensitive autologous Envs from the VRC01 donor revealed no significant difference in repli- cation kinetics or efficiency

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