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Developmental program expression of myosin alkali light chain and skeletal actin genes in the rainbow troutOncorhynchus mykiss1 The sequence data in this paper have been submitted to the EMBL/GenBank data libraries under the accession numbers AF330140, AF330141, AF330142 and AF360980.1

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
0167-4781
Publisher
Elsevier
Publication Date
Volume
1519
Identifiers
DOI: 10.1016/s0167-4781(01)00221-4
Keywords
  • Myosin Alkali Light Chain
  • Skeletal Actin
  • Cdna
  • Skeletal Muscle
  • Embryo
  • Rainbow Trout

Abstract

Abstract We have isolated MLC1 F (tMLC1 F), MLC3 F (tMLC3 F) and skeletal actin cDNAs from the teleost Oncorhynchus mykiss. Sequence analysis indicates that tMLC1 F and tMLC3 F are not produced from differentially spliced mRNAs as reported in avians and rodents but are encoded by different genes. Results from RNase protection analysis showed that the corresponding transcripts are expressed in fast skeletal muscles. Whole-mount in situ hybridisation revealed distinct expression patterns of the myosin alkali light chains and skeletal actin genes during skeletal muscle development in the embryo.

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