Affordable Access

Publisher Website

Electrode measurement of oxygen tension with 1-ms time resolution

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Bioenergetics
0005-2728
Publisher
Elsevier
Publication Date
Volume
396
Issue
2
Identifiers
DOI: 10.1016/0005-2728(75)90030-4

Abstract

Abstract A continuous flow device utilizing a Clark oxygen electrode was constructed; this device had a dead time and resolution of 1 ms. Mixing was tested by observing the neutralization of acid with base, and at the maximal flow rate, the mixing was 94% complete within 1 ms and better than 98% complete within 2 ms after initial mixing. Observation of the oxygenation of hemoglobin gave data which agreed with previous data obtained by a stopped-flow optical experiment. The respiration of phosphorylating submitochondrial particles was measured utilizing this device. The burst of respiration in submitochondrial particles was triphasic, with a very rapid burst lasting some 60 ms, followed by a longer burst of respiration lasting more than 4 s.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Electrode measurement of oxygen tension with 1-ms...

on Biochimica et Biophysica Acta Aug 11, 1975

An extremely rapid burst of respiration upon aerat...

on Archives of Biochemistry and B... June 1972

An extremely rapid burst of respiration upon aerat...

on Archives of Biochemistry and B... Jan 01, 1972
More articles like this..