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α-d-Mannosidases in serum of patients with I-cell disease (ICD)

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
112
Issue
2
Identifiers
DOI: 10.1016/0009-8981(81)90374-0

Abstract

Abstract The specific activity of α- d-mannosidase m serum of ICD-patients is considerably increased due to increased amounts of the component with optimal activity at pH 4.6 (acidic form). The intermediate form with pH optimum of 6.0 remains unaltered. These conclusions were reached by using optimal conditions for differential assay of the α- d-mannosidases checked by partial separation of the components in serum by sucrose gradient centrifugation.

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