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Systematic identification of novel SLE related autoantibodies responsible for type I IFN production in human plasmacytoid dendritic cells

Authors
Journal
Cellular Immunology
0008-8749
Publisher
Elsevier
Volume
284
Identifiers
DOI: 10.1016/j.cellimm.2013.07.017
Keywords
  • Protein Array
  • Autoimmune
  • Innate Immunity
  • Sle
  • Plasmacytoid Dendritic Cells
  • Type I Interferons
Disciplines
  • Biology

Abstract

Abstract Plasmacytoid dendritic cells [pDC], also known as type I interferon [IFN] producing cells, play a significant role in the pathogenesis of systemic lupus erythematosus [SLE]. The current study was undertaken to identify novel SLE autoantibody specificities associated with interferon-inducing activity in human pDCs. We found that immune complex mixtures from some Interferon signature negative [IFN−] and all interferon signature positive [IFN+] SLE patients could trigger type I IFN production by pDCs. IgGs from IFN− and IFN+ SLE patients were subsequently screened via a high throughput protein microarray to identify novel auto-antibody specifities that mediate type I IFN production by pDCs. This approach identified five novel autoantibodies that may contribute to type I IFN production by pDCs via a nucleic acid dependent mechanism. The newly identified autoantibody specificities function in a myriad of cell processess and, to date, have not been implicated in SLE pathogenesis.

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