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Automated determination of enzymatic rate functions by the ‘gradient-flow’ method

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
51
Issue
2
Identifiers
DOI: 10.1016/0003-2697(73)90514-9
Disciplines
  • Biology

Abstract

Abstract Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A general criterion for safeguarding the validity of rate functions recorded by this method has been proposed.

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