Abstract Aboard the Huygens probe, which descended through Titan's atmosphere in January 2005, was the Surface Science Package (SSP), a set of 9 sensors, including a speed-of-sound sensor. We present the first detailed description of the SSP speed of sound measurements and report constraints on the methane content in Titan's lower atmosphere based on these measurements. After a careful calibration and subsequent Bayesian analysis, our measurements yield a most likely methane fraction in Titan's lower atmosphere of approximately 2% at 10 km, increasing to 3.5% at lower altitudes, based on a binary composition. Our data show that any large scale variation of methane within the lower 11 km of Titan's atmosphere is unlikely. Within experimental and theoretical uncertainties, our results are compatible with earlier estimates obtained from the GCMS experiment [Niemann, H.B., Atreya, S.K., Bauer, S.J., Carignan, G.R., Demick, J.E., Frost, R.L., Gautier, D., Haberman, J.A., Harpold, D.N., Hunten, D.M., Israel, G., Lunine, J.I., Kasprzak, W.T., Owen, T.C., Paulkovich, M., Raulin, F., Raaen, E., Way, S.H., 2005. Nature 438 (7069), 779–784].