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Nitrite binding to cytochrome P-450 from liver microsomes of trout (salmo gairdneririch.) and effects on two microsomal enzymes

Authors
Journal
Toxicology Letters
0378-4274
Publisher
Elsevier
Publication Date
Volume
21
Issue
3
Identifiers
DOI: 10.1016/0378-4274(84)90099-7
Keywords
  • Nitrite Toxicity
  • Trout Liver Microsomes
  • Cytochrome P-450-No Complex
  • Cytochrome P-420-No Complex
  • Aminopyrine Demethylase
  • Dimethylaniline Monooxygenase
Disciplines
  • Biology

Abstract

Abstract Addition of nitrite to dithionite-reduced trout liver microsomes leads to the conversion of cytochrome P-450 into a cytochrome P-420-NO complex, as it does in mammalian microsomes. A loss in cytochrome P-450 and an inhibition of aminopyrine demethylase (AP) activity were observed in vitro at nitriteconcentrations found in the liver of trout exposed in vivo to this toxin. Nitrite had no effect on dimethylaniline monooxygenase (DMA), a cytochrome P-450-independent enzyme.

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