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Simultaneous determination of pyridoxal and pyridoxamine by different spectrofluorimetric techniques

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
42
Issue
1
Identifiers
DOI: 10.1016/0039-9140(94)00228-k

Abstract

Abstract Binary mixtures of pyridoxal and pyridoxamine can be resolved by using zero-crossing first derivative spectrofluorimetry, first devivative constant wavelength synchronous luminescence spectrometry and first derivative constant energy synchronous luminescence spectrometry. These methods do not require any previous separation steps. The lowest quantization limit is obtained with first derivative constant energy synchronous fluorescence (13.0 and 9.0 μg/1. for pyridoxal and pyridoxamine, respectively). The measurements were performed in aqueous medium at pH 7.0 provided by adding 0.05 M phosphate buffer solution. In order to demonstrate the validity of these methods a complete and exhaustive statistical analysis of the experimental data was performed. Pyridoxal and pyridoxamine were determined by these methods in synthetic and real mixtures with good results.

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