Affordable Access

Publisher Website

Immunocytochemical Detection ofRhodococcus equiin Tracheal Washes of Foals

Authors
Journal
Journal of Comparative Pathology
0021-9975
Publisher
Elsevier
Publication Date
Volume
145
Issue
1
Identifiers
DOI: 10.1016/j.jcpa.2010.11.014
Keywords
  • Foal
  • Tracheal Wash
  • Immunocytochemistry
  • Rhodococcus Equi
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Summary The aim of the present study was to develop an immunocytochemical procedure for the early detection and demonstration of Rhodococcus equi in smears of tracheal aspirates taken from live foals in field conditions. Tracheal wash samples were collected from thoroughbred foals, aged 1–5 months and located in studs around Bursa and Istanbul, Turkey. Some foals were suspected of having R. equi infection on the basis of clinical examination ( n = 56) and others were unaffected control animals ( n = 54). Serum samples were also collected from each foal for testing for the presence of R. equi-specific antibody by enzyme linked immunosorbent assay (ELISA). Thirty-six of the control foals (66.7%) and 37 of the affected foals (66.1%) were seropositive for R. equi. Immunocytochemical labelling was detected in the smears from 73.2% of the affected foals and 70.4% of the control foals. For both ELISA and immunocytochemistry (ICC), there was no significant difference between the affected and control foals ( P > 0.05) and there was no significant difference between the two test modalities ( P > 0.05). ICC may therefore have similar diagnostic utility when compared with ELISA. There is no clear relationship between clinical signs and ELISA or ICC positivity.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Role of antibody to extracellular proteins ofRhodo...

on Veterinary Microbiology Jan 01, 1991

A serological survey ofRhodococcus equiinfection i...

on Comparative Immunology Microbi... Jan 01, 2003

Pathogenicity and virulence ofRhodococcus equiin f...

on Veterinary Microbiology Jan 01, 1997

Diagnosis ofRhodococcus equiinfection in foals by...

on Veterinary Microbiology Jan 01, 1987
More articles like this..