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Citrate Lyase: A Pantothenate-Containing Enzyme

Authors
Publication Date
Source
PMC
Keywords
  • Biological Sciences: Biochemistry
Disciplines
  • Biology

Abstract

Citrate lyase from Klebsiella aerogenes inactivated by reaction in the presence of substrate or by treatment with hydroxylamine can be reactivated with acetic anhydride only if its sulfhydryl groups are reduced. Alkaline hydrolysis of pure citrate lyase yields about 3 mol of phosphopantothenate per mol of enzyme.

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