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Electron microscopical identification of 3,3′,5,5′-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
23
Issue
1
Identifiers
DOI: 10.1016/0304-3940(81)90181-6
Disciplines
  • Chemistry

Abstract

Abstract Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB-HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB-HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.

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