Affordable Access

Publisher Website

Interleukin-10 protects against lethality of intra-abdominal infection and sepsis

Authors
Journal
Journal of Gastrointestinal Surgery
1091-255X
Publisher
Springer-Verlag
Publication Date
Volume
4
Issue
1
Identifiers
DOI: 10.1016/s1091-255x(00)80035-9
Keywords
  • Interleukin-10
  • Sepsis
  • Tumor Necrosis Factor (Tnf)
  • Intra-Abdominal Infection/Abscess
Disciplines
  • Biology

Abstract

Abstract The aim of this study was to determine whether interleukin-10 would alter locally derived and systemic proinflammatory cytokine expression and protect from the lethality of cecal ligation and puncture. Three groups of Sprague-Dawley rats were used. Group 1 underwent cecal manipulation. Groups 2 and 3 underwent cecal ligation and puncture. Group 2 received intraperitoneal saline injections beginning 1 hour after cecal ligation and puncture and every 3 hours thereafter for 24 hours. Group 3 received intraperitoneal interleukin-10 one hour after cecal ligation and puncture and every 3 hours thereafter. Animals were killed at 6 and 24 hours after cecal ligation and puncture or sham operation. Serum tumor necrosis factor-alpha (TNF-α) levels were determined by enzyme-linked immunosorbent assay. TNF-α messenger RNA expression was determined by reverse transcriptase—polymerase chain reaction using β-actin as the internal standard. There was a twofold increase ( P <0.001) in TNF-α mRNA in the liver at 6 and 24 hours after cecal ligation and puncture when compared to rats treated with interleukin-10. There was a twofold increase ( P <0.05) in TNF-α mRNA in the lung observed only at 24 hours after cecal ligation and puncture when compared to rats treated with interleukin-10. Serum levels of TNF-α were elevated at 6 hours in control animals, and this effect was abolished by the administration of interleukin-10. There was no difference in mortality rates at 6 hours (0% for all groups); however, at 24 hours 57% (4/7) mortality was observed in group 2 vs. 0% (0/20) in groups 1 and 3. Interleukin-10 given after the onset of cecal ligation and puncture protects against the lethality of intra-abdominal sepsis.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Interleukin-10 protects against lethality of intra...

on Journal of Gastrointestinal Su... 2000

Interleukin-10 Prevents Early Cytokine Release in...

on Journal of Surgical Research Jan 01, 1997

Interleukin-10 prevents early cytokine release in...

on Journal of Surgical Research Jul 01, 1997
More articles like this..