Affordable Access

IGF-1 regulates apoptosis of cardiac myocyte induced by osmotic-stress

Biochemical and Biophysical Research Communications
Publication Date
  • Insulin-Like Growth Factor-1
  • Osmotic Stress
  • Doxorubicin
  • Growth-Factor-I
  • Activated Protein-Kinase
  • Renin-Angiotensin System
  • Signal-Transduction
  • Ventricular Hypertrophy
  • Transgenic Mice
  • Rat Cardiomyocytes
  • Gene-Expression


Insulin-like growth factor-1 (IGF-1) is a natural protectant of cardiac myocytes that has been shown to improve cardiac function. The role of IGF-1 in attenuating apoptosis induced by osmotic stress (sorbitol, SOR) or by other known apoptotic stimuli (doxorubicin, angiotensin II, and serum withdrawal) was determined in cultured cardiac myocytes. After 6 h of exposure to SOR, apoptosis was initiated, concomitant with a decrease in cell survival and increases in poly[ADP-ribose] polymerase (PARP) degradation and DNA fragmentation. These effects were maximal after 24 h. IGF-1 partially attenuated apoptosis induced by sorbitol but not that induced by angiotensin II, doxorubicin, or serum withdrawal. In cells preincubated with IGF-1 before the addition of SOR, we detected an increase in the number of viable cells, a decrease in the generation of DNA fragments on agarose gel electrophoresis and in the percentage of positive TUNEL cells, and a reduction on PARP levels. These results suggest that IGF-1 prevents apoptosis induced by osmotic stress in cardiac myocytes but not apoptosis induced by doxorubicin and angiotensin II.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times