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The presence ofd-β-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
294
Issue
5
Identifiers
DOI: 10.1016/s0006-291x(02)00597-1
Keywords
  • Aging
  • D-Aspartic Acid
  • β-Aspartic Acid
  • Elastic Fiber
  • Immunohistochemistry
  • Sun Damage
  • Skin
Disciplines
  • Biology

Abstract

Abstract Biologically uncommon d-aspartyl residues have been reported in proteins of various elderly tissues. We prepared a polyclonal antibody against d-β-Asp-containing peptide and examined its immunoreactivity in the skin. The antibody recognized integrated or disintegrated elastic fibers in the sun-exposed skin but not in the sun-protected skin of the elderly donors. Western blot analysis of the proteins isolated from sun-damaged skin demonstrated that the 50 kDa protein was immunoreactive with both antibodies for d-β-Asp-containing peptide and elastin. Ultraviolet (UV) irradiation on normal skin caused the appearance of d-β-Asp-containing peptide-immunoreactive fibers in the dermis. These results suggest that UV irradiation is closely related to the formation of d-β-Asp in the elastic fibers of skin. We propose that the antibody could be a useful indicator for sun damage of the skin.

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