Abstract As a continuation of our previous study in TLC line ( 1, 6) we shall consider the TLC separation of estrone its ethers and acetate as well as their oximes and their rearrangement products namely the D-homo-aza-lactams. Comparative examinations and structural identification of homo-aza-lactams occurring in Beckmann rearrangement and Schmidt reaction were described in some of our earlier publications ( 5, 7). The D-homo-aza-lactams derived from the esterone and estrone 3-methyl ether, were described by Regan and Hayes ( 9) and we too, carried out the completion of these examinations ( 4). Numerous papers, summaries, and books ( 2, 3, 8) describe the TLC separation of estrone and its derivatives occurring in nature due to their biological importance. Consequently our paper can furnish a completion from a preparative organic chemical viewpoint concerning the separation of the above mentioned rearrangement products.