Interferon-α/β receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006

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Interferon-α/β receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006

Authors
Publisher
BioMed Central
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PMC
Keywords
  • Research Article

Abstract

1471-2172-4-8.fm ral ss BioMed CentBMC Immunology Open AcceResearch article Interferon-α/β receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006 Atsushi Kato1,2, Toshiki Homma1,2, Jonathan Batchelor3, Noriko Hashimoto1, Shosuke Imai4, Hiroshi Wakiguchi5, Hirohisa Saito1,6 and Kenji Matsumoto*1,6 Address: 1Department of Allergy & Immunology, National Research Institute for Child Health & Development, Tokyo, Japan, 2Genox Research, Inc., Kawasaki, Japan, 3Division of Allergy, National Center for Child Health and Development, Tokyo, Japan, 4Department of Microbiology, Kochi Medical School, Kochi, Japan, 5Department of Pediatrics, Kochi Medical School, Kochi, Japan and 6Research Team for Allergy Transcriptome, RIKEN Research Center for Allergy & Immunology, Yokohama, Japan Email: Atsushi Kato - [email protected]; Toshiki Homma - [email protected]; Jonathan Batchelor - [email protected]; Noriko Hashimoto - [email protected]; Shosuke Imai - [email protected]; Hiroshi Wakiguchi - wakiguti@med.kochi- ms.ac.jp; Hirohisa Saito - [email protected]; Kenji Matsumoto* - [email protected] * Corresponding author Abstract Background: Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) are known to exert a strong adjuvant effect on Th1 immune responses. Although several genes have been reported, no comprehensive study of the gene expression profiles in human cells after stimulation with CpG ODN has been reported. Results: This study was designed to identify a CpG-inducible gene cluster that potentially predicts for the molecular mechanisms of clinical efficacy of CpG ODN, by determining mRNA expression in human PBMC after stimulation with CpG ODN. PBMCs were obtained from the peripheral blood of healthy volunteers and cultured in the presence or absence of CpG ODN 2006 for up to 24 hours. The mRNA expression profile was evaluated using a high-density oligonucleotide probe array, GeneChip®. Using hierarchical clustering-a

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