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A novel, inexpensive, and sensitive method for analysis of tyrosine hydroxylase activity in tissue samples

Authors
Journal
Life Sciences
0024-3205
Publisher
Elsevier
Publication Date
Volume
18
Issue
8
Identifiers
DOI: 10.1016/0024-3205(76)90013-8
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Tyrosine hydroxylase activity in whole mouse brains was measured in v vitro . The L-dihydroxyphenylaline (L-DOPA) formed by the enzyme was quantitated by liquid chromatography with electrochemical detection (LCEC). An investigation of the incubation factors (added Fe +2, DOPA decarboxylase inhibitor concentration, substrate concentration, amount of tissue, time of incubation) is reported. Under optimal conditions the activity was found to be 15.1 ± 0.6 (S.E.M.) nmol DOPA formed/hr./g. tissue.

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