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Microbial conversion of dihydrooxoisophorone (DOIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) by thermophilic bacteria

Authors
Journal
Journal of Biotechnology
0168-1656
Publisher
Elsevier
Publication Date
Volume
9
Issue
2
Identifiers
DOI: 10.1016/0168-1656(89)90081-3
Keywords
  • Thermophilic Bacterium
  • Dihydrooxoisophorone
  • 4-Hydroxy-2
  • 2
  • 6-Trimethylcyclohexanone
  • Air-Lift Bioreactor

Abstract

Abstract Dihydrooxoisophorone (DOIP, 2,2,6-trimethyl-1,4-cyclohexanedione) was converted to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) by a thermophile isolated from soil. The thermophile was identified as Bacillus stearothermophilus. 4-HTMCH has four isomers: cis-(4 R, 6 S)-, cis-(4 S, 6 R)-, trans-(4 R, 6 R)- and trans-(4 S, 6 S)-. Their quantitative ratio was 68:25:5:2. The extent of DOIP conversion was 75% in batch culture after 20 h cultivation and 73% in continuous culture at a dilution rate of 0.07 h −1 in an air-lift reactor. The maximum production rate of 4-HTMCH in continuous culture in an air-lift reactor was 200 mg l −1 h −1 at a dilution rate of 0.12 h −1. This microbial conversion was closely associated with cell growth. The relationship between conversion of DOIP and cell growth was also investigated.

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