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A reproducible method for the optimum infection of human mononuclear cells and lymphoid cell lines by herpes simplex virus type I

Authors
Journal
Journal of Virological Methods
0166-0934
Publisher
Elsevier
Publication Date
Volume
4
Issue
2
Identifiers
DOI: 10.1016/0166-0934(82)90077-5
Keywords
  • Cell Surface Antigens
  • Herpes Simplex Virus Type 1
  • Infection Of Cells

Abstract

Abstract Parameters for the infection of human mononuclear cells (MNC) with herpes simplex virus type 1 (HSV-1) were investigated and a procedure was established which resulted in a reproducible optimum number of cells expressing virus-specific cell surface antigens The number of cells infected was independent of the sex of the donor and independent of whether the donor was HSV- seropositive or -seronegative. On the average 18±6% of HSV-infected MNC from any given donor expressed HSV-specific cell surface antigens. When the standard procedure was applied to a variety of lymphoid cell lines, a high percentage of cells of both B and non-T/non-B lines expressed HSV-specific cell surface antigens, whereas T-cell lines appeared resistant to HSV infection.

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