Affordable Access

Publisher Website

Influence of ionic strength on the binding of sodium aurothiosulphate to human serum albumin

Authors
Journal
Biochemical Pharmacology
0006-2952
Publisher
Elsevier
Publication Date
Volume
34
Issue
24
Identifiers
DOI: 10.1016/0006-2952(85)90291-6

Abstract

Abstract The effect of ionic strength on the binding of aurothiosulphate to human serum albumin has been studied at 37° and neutral pH by equilibrium dialysis in unbuffered solutions. The effect of ionic strength is more pronounced on the lower association constants K 2– K 4 than on the high association constant K 1. Furthermore a reduction in the number of lower affinity binding sites is observed at low ionic strength. The main ionic strength dependence on the association constants agrees with the Debye-Hückel theory. The extrapolated values of K 1 and the sum of K 2 to K 4 at zero ionic strength are 7.6 × 10 5M −1 and 1.1 × 5M −1, respectively. It is shown that the observed changes in pH of the albumin solutions during dialysis contains valuable information of the aurothiosulphate-albumin interaction. A molecular binding mechanism is discussed.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Influence of ionic strength on the binding of sodi...

on Biochemical Pharmacology Dec 15, 1985

Influence of temperature on the binding of sodium...

on Biochemical Pharmacology Jul 15, 1986

Influence of temperature on the binding of sodium...

on Biochemical Pharmacology Jan 01, 1986

Binding of digitoxin to human serum albumin: influ...

on Acta pharmacologica et toxicol... November 1975
More articles like this..