Abstract Uniform Design was used to optimize the effects of processing parameters for extraction of polysaccharides from Fructus Corni. Four independent variables including extraction temperature (°C), extraction time (min), liquid ratio (times) and pH value were studied. The optimal conditions were determined and tri-dimensional response surfaces were plotted according to the try grid method. The results indicated that liquid ratio (times) and pH value variables markedly affect the polysaccharides value. Relevant parameters of the extract of the highest concentration of polysaccharide are as follows that the liquid ratio is 1: 33 with the pH value of 8.5. Under optimized conditions the experimental yield was in agreement with the predicted value.