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Corneal epithelial Cl-dependent pump quantified

Authors
Journal
Experimental Eye Research
0014-4835
Publisher
Elsevier
Publication Date
Volume
43
Issue
5
Identifiers
DOI: 10.1016/s0014-4835(86)80002-1
Keywords
  • Cornea
  • Epithelium
  • Active Fluid Pump

Abstract

Pairs of rabbit corneas were perfused and corneal thickness monitored. The epithelial side of the preparation, and after stabilization also the endothelial side, was covered with silicone oil. A constant thinning of the cornea was observed at a rate of 11·1 μm hr −1 (= 1·0 μm L h −1 cm −2) s.d. 2·1, n = 9. This thinning was also present after endothelial removal: 12·3 μm hr −1, s.d. 1·6 ( n = 5). Epithelial abrasion virtually abolished the thinning (2·5 μm hr −1, s.d. 1·89, n = 4), as did cooling from 34 to 0°C. When the preparation was perfused with a Cl-free solution (SO 4 2− instead of Cl −, corrected for osmolarity with sucrose) no significant thinning of the preparation was observed (2·4 μm hr −1, s.d. 2·49, n = 4) after covering both surfaces with silicone oil. This simple set of experiments quantified the epithelial pump mechanism. The epithelial pump rate of about 1·2 μm L hr −1 cm −2 has to be taken into account when endothelial pump rates are measured in the in vitro preparation with intact epithelium.

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