Affordable Access

Publisher Website

Determination of acrolein by headspace solid-phase microextraction gas chromatography and mass spectrometry

Authors
Journal
Journal of Chromatography B Biomedical Sciences and Applications
0378-4347
Publisher
Elsevier
Publication Date
Volume
758
Issue
1
Identifiers
DOI: 10.1016/s0378-4347(01)00152-9
Keywords
  • Acrolein

Abstract

Abstract We developed a headspace solid-phase microextraction (headspace SPME) method to measure acrolein in human urine. This new technique resolves some problems with the headspace gas chromatography and mass spectrometry (GC–MS) method which we developed previously. With the original method, a column and a filament were damaged by the injection of air. A 0.5-ml urine (or phosphate-buffered saline) sample in a glass vial containing propionaldehyde as an internal standard was heated for 5 min. The SPME fiber (65 μm carbonwax–divinylbenzene fiber) was exposed to the headspace and then inserted into a GC–MS instrument in which a DB-WAX capillary column (30 m×0.32 mm, film thickness 0.5 μm) was installed. The total analysis time was 15 min. The inter-assay and intra-assay coefficients of variation were 10.07 and 5.79%, respectively. The calibration curve demonstrated good linearity throughout concentrations ranging from 1 to 10 000 n M. The headspace SPME method exhibits high sensitivity and requires a short analysis time as well as the previous method. We conclude that this method is useful to measure urinary acrolein.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Determination of acrolein by headspace solid-phase...

on Journal of chromatography. B,... Jul 05, 2001

Determination of acrolein in french fries by solid...

on Journal of Chromatography A Jan 01, 2011

[Determination of five synthetic musks in perfume...

on Se pu = Chinese journal of chr... February 2012

Determination of volatile oak compounds in wine by...

on Journal of Chromatography A Jan 13, 2006
More articles like this..