Affordable Access

MIGRATION OF GLYCOPROTEIN FROM GOLGI APPARATUS TO CELL COAT IN THE COLUMNAR CELLS OF THE DUODENAL EPITHELIUM

Authors
Journal
The Journal of Cell Biology
0021-9525
Publisher
The Rockefeller University Press
Publication Date
Keywords
  • Brief Notes
  • Article
Disciplines
  • Biology
  • Chemistry

Abstract

668.tif MIGRATION OF GLYCOPROTEIN FROM GOLGI APPARATUS TO CELL COAT IN THE COLUMNAR CELLS OF THE DUODENAL EPITHELIUM GARY BENNETT. From the Department of Anatomy, MeGill Univel~sity, Montreal 112, Canada A layer of carbohydrate material appears at the surface of a variety of mammalian cells (1) and is particularly abundant at free surfaces such as the striated border of the columnar cells of the intestine (2). In electron micrographs of intestinal cells, this material appears as a discrete coating covering the tips and sides of the microvilli (3). From the various histochemical tests applied (3-I0), it has been concluded that this "cell coat" consists of glycoprotein rich in acidic residues (I, 9). After incubation of cat intestine with a labeled precursor of complex carbohydrates (glucose-SH) followed by EM radioautography, Ito (l l) ob- served radioautographic reaction first over the cytoplasm of columnar cells and, after 1 hr, over the cell coat of the free surfaces. In this laboratory, by means of light microscopic radioautography, after injection of galactose-3H into young rats, label was localized, at early time intervals, to the Golgi region of duodenal columnar cells, and later traced 668 B R I E F N O T E S to the striated border (12, 13). Jersild (14) con- firmed this early localization of galactose-SH to the Golgi apparatus of intestinal cells with the electron microscope. In recent in vitro EM radio- autographic studies, Ito (15) reported incorpora- tion of tritium-labeled mannose and galactose into the Golgi apparatus of cat intestinal cells at early time intervals and into the apical surface coat later. The present in vivo study, on young rats, is an attempt to locate more exactly the source of apical cell coat material in rat duodenal columnar cells, and to learn about its mode of transport to the cell surface. MATERIALS AND METHODS Single injections of galactose-SH (3.33-5.0 mCi/ animal; specific activity 1.17-

There are no comments yet on this publication. Be the first to share your thoughts.