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Modification of bacteriophage with hapten- ϵ-aminocaproyl-N-hydroxysuccinimide esters; Increased sensitivity for immunoassay

Authors
Journal
Immunochemistry
0019-2791
Publisher
Elsevier
Publication Date
Volume
12
Issue
4
Identifiers
DOI: 10.1016/0019-2791(75)90184-6

Abstract

Abstract Batches of bacteriophage T4 were conjugated with one of the haptens: NIP, NP or DNP by inserting an ϵ-aminocaproyl spacer between the hapten and the phage. The three haptencaproyl phages were approximately ten times more sensitive to anti-hapten antibodies than the corresponding directly haptenated phages with an optimal hapten coat. The hapten-caproyl phages could detect immune anti-hapten antibody (50 per cent inactivation) at concentrations ranging from 17 to 90 pg/ml and the one tested batch of natural anti-NIP at a concentration of 210 pg/ml. They can be used for the quantitation of IgM (and perhaps IgA) in body fluids at concentrations as low as 100 ng/ml.

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