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A new, sensitive determination of phosphate

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
30
Issue
1
Identifiers
DOI: 10.1016/0003-2697(69)90372-8

Abstract

Abstract A new method for the determination of phosphorus using Triton X-100 instead of reducing agents is more sensitive than procedures described earlier. Organic phosphate esters do not appear to interfere with the determination of free phosphate under the conditions of the assay. All reagents are stable at room temperature and the absorbance is measured at convenient wavelengths.

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