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Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
8
Issue
4
Identifiers
DOI: 10.1371/journal.pone.0062760
Keywords
  • Research Article
  • Biology
  • Anatomy And Physiology
  • Musculoskeletal System
  • Musculoskeletal Anatomy
  • Computational Biology
  • Genomics
  • Genome Expression Analysis
  • Molecular Genetics
  • Gene Expression
  • Developmental Biology
  • Histology
  • Model Organisms
  • Animal Models
  • Mouse

Abstract

The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice.

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