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Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12

Authors
Publisher
American Society of Plant Biologists
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology
  • Chemistry

Abstract

Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12 by Jan Smalle, Jasmina Kurepa, Peizhen Yang, Elena Babiychuk, Sergei Kushnir, Adam Durski, and Richard D. Vierstra Plantcell Volume 14(1):17-32 January 1, 2002 ©2002 by American Society of Plant Biologists Organization and Derived Amino Acid Sequence Comparisons of the Arabidopsis RPN12a and RPN12b Genes and Association of the RPN12a Protein with the 26S Proteasome.(A) Genomic organization of Arabidopsis RPN12a and predicted RPN12b. Smalle J et al. Plantcell 2002;14:17-32 ©2002 by American Society of Plant Biologists Molecular and Biochemical Descriptions of the Arabidopsis rpn12a-1 Mutation.(A) Scheme of the RPN12a gene and the predicted protein product of rpn12a-1, a RPN12a-NTPII translational fusion protein. Smalle J et al. Plantcell 2002;14:17-32 ©2002 by American Society of Plant Biologists Association of the RPN12a-1–NPTII Fusion Protein with the 26S Proteasome.The 26S proteasome was partially purified from Arabidopsis seedlings homozygous for the rpn12a-1 mutation by sequential polyethylene glycol precipitations and subjected to size exclusi... Smalle J et al. Plantcell 2002;14:17-32 ©2002 by American Society of Plant Biologists Developmental Changes Induced by the rpn12a-1 Mutation.(A) Increased accumulation of anthocyanins in hypocotyls of 1-week- old rpn12a-1 seedlings. Smalle J et al. Plantcell 2002;14:17-32 ©2002 by American Society of Plant Biologists The rpn12a-1 Mutant Has Decreased Sensitivity to the Cytokinins Kinetin, BA, and 2iP.(A) Shoot growth inhibition and chlorosis induced by kinetin is stronger in wild-type (WT) than in rpn12a-1 seedlings. Smalle J et al. Plantcell 2002;14:17-32 ©2002 by American Society of Plant Biologists Expression of Specific Cytokinin-Inducible Genes Is Altered by the rpn12a-1 Mutation.Wild-type (WT) and rpn12a-1 seedlings were grown in liquid medium for 14 days and then treated with the cytokinin kinetin or BA. After the treatments, total RNA was extr

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