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Reductive nitrosylation ofMethanosarcina acetivoransprotoglobin: A comparative study

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
430
Issue
4
Identifiers
DOI: 10.1016/j.bbrc.2012.11.122
Keywords
  • Methanosarcina Acetivoransprotoglobin
  • Reductive Nitrosylation Of Ferricmethanosarcina Acetivoransprotoglobin
  • Nitrosylation Of Ferrousmethanosarcina Acetivoransprotoglobin
  • Kinetics
  • Thermodynamics
Disciplines
  • Physics

Abstract

Abstract Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O2 detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, M. acetivorans Pgb (MaPgb) binds preferentially O2 rather than CO and displays anticooperativity in ligand binding. Here, kinetics and/or thermodynamics of ferric and ferrous MaPgb (MaPgb(III) and MaPgb(II), respectively) nitrosylation are reported. Data were obtained between pH 7.2 and 9.5, at 22.0°C. Addition of NO to MaPgb(III) leads to the transient formation of MaPgb(III)–NO in equilibrium with MaPgb(II)–NO+. In turn, MaPgb(II)–NO+ is converted to MaPgb(II) by OH−-based catalysis. Then, MaPgb(II) binds NO very rapidly leading to MaPgb(II)–NO. The rate-limiting step for reductive nitrosylation of MaPgb(III) is represented by the OH−-mediated reduction of MaPgb(II)–NO+ to MaPgb(II). Present results highlight the potential role of MaPgb in scavenging of reactive nitrogen and oxygen species.

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