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Prostaglandin concentrations and prostaglandin synthetase activity inN-nitrosomethylurea-induced rat mammary adenocarcinoma

Authors
Journal
European Journal of Cancer and Clinical Oncology
0277-5379
Publisher
Elsevier
Publication Date
Volume
19
Issue
6
Identifiers
DOI: 10.1016/0277-5379(83)90014-7
Disciplines
  • Biology

Abstract

Abstract A comparison of tissue concentrations and biosynthesis of prostaglandin (PG)E 2, PGF 2α, 6-keto-PGF 1α (degradation production of PGI 2) and thromboxane (TX)B 2 (degradation product of TXA 2) was made in normal mammary glands obtained from virgin female Sprague-Dawley rats and in N-nitrosomethylurea (NMU)-induced mammary adenocarcinomas. The tissue concentrations ( ng g wet weight) of all 4 compounds were significantly higher in the NMU-induced tumor than in normal mammary tissue: PGE 2, 210 ± 37 vs 25 ± 6; PGE 2α, 287 ± 48 vs 23 ± 8; 6-keto-PGF 1α, 294 ± 42 vs 31 ± 8; and TXB 2, 260 ± 49 vs 27 ± 5 (mean ± S.E.M.). Microsomal prostaglandin synthetase activity in NMU-induced tumors was also significantly higher than in normal tissue for all but 6-keto-PGF 1α: PGE 2, 226 ± 16 vs 50 ± 9; PGF 2α, 28 ± 3 vs 4 ± 1; 6-keto-PGF 1α, 14 ± 2 vs 11 ± 2; and TXB 2, 17 ± 1 vs 10 ± 1 ng/mg protein (mean ± S.E.M.). There was no apparent relationship between either tumor size or age and the ability of microsomal enzyme to synthesize prostaglandins, although the content of prostaglandins extracted from tumor tissue was inversely related to the tumor size.

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