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Development of an inducible system to control and easily monitor gene expression inLactococcus lactis

Authors
Journal
Plasmid
0147-619X
Publisher
Elsevier
Publication Date
Volume
51
Issue
3
Identifiers
DOI: 10.1016/j.plasmid.2004.02.004
Keywords
  • Lactococcus Lactis
  • Expression Vector
  • Ribonuclease Iii
  • Maltose-Induction System
  • Regulation Of Gene Expression

Abstract

Abstract This report describes the implementation and use of a maltose-inducible system for regulated gene expression in Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector can be easily monitored by fluorescence spectroscopy and microscopy. As an example, the lactococcal ribonuclease III was overproduced in an active form.

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