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Analysis of glycoproteins in cell culture supernatants using a lectin immunosensor technique

Authors
Journal
Biosensors and Bioelectronics
0956-5663
Publisher
Elsevier
Publication Date
Volume
17
Issue
10
Identifiers
DOI: 10.1016/s0956-5663(02)00111-2
Keywords
  • Surface Plasmon Resonance
  • Glycosylation
  • α1-Acid Glycoprotein
  • Lectins
  • Cytokines
Disciplines
  • Biology

Abstract

Abstract A method based on a surface plasmon resonance technique for detection of changes in concentration and glycosylation of proteins in cell culture supernatant is described. The method was used to analyze α 1-acid glycoprotein (AGP) produced by a human hepatoma cell line (HepG2). Cell culture supernatant was injected to a BIACORE 2000 instrument and AGP was captured on the sensor chip by immobilized antibodies. The captured glycoprotein was then analyzed for content of carbohydrate epitopes using three different lectins, Aleuria aurantia lectin (AAL), Sambucus nigra agglutinin (SNA), and Triticum vulgaris agglutinin (wheat germ agglutinin, WGA). The method was used to analyze changes in concentration and glycosylation of AGP produced by HepG2 cells grown with or without three different cytokines, interleukin-1β (IL-1β), interleukin-6 (IL-6), and transforming growth factor β-1 (TGFβ 1). Using the described method it was shown that when HepG2 cells were grown in the presence of IL-6 both AGP concentration and fucosylation increased. When HepG2 cells instead were grown in the presence of TGFβ 1 AGP fucosylation increased whereas AGP concentration decreased.

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