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High level of expression and stability of the cloned alkaline protease (aprA) gene inBacillus subtilis

Authors
Journal
Enzyme and Microbial Technology
0141-0229
Publisher
Elsevier
Publication Date
Volume
16
Issue
6
Identifiers
DOI: 10.1016/0141-0229(94)90026-4
Keywords
  • Bacillus Subtilis
  • Alkaline Protease
  • Plasmid Stability

Abstract

Abstract Gene expression and plasmid stability of the cloned alkaline protease ( aprA) gene in Bacillus subtilis were investigated. B. subtilis cells harboring the multicopy aprA gene were grown on sporulation medium and the activity of the alkaline protease was monitored throughout the cultivation time. Results presented indicate that the expression of the aprA gene occurred late during the stationary phase and the plasmid that carries the aprA gene was segregationally and structurally stable.

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