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The simultaneous fluorimetric microdetermination of cortisol and corticosterone in plasma

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
6
Issue
5
Identifiers
DOI: 10.1016/0009-8981(61)90116-4
Disciplines
  • Medicine

Abstract

Abstract 1. (1) A simplified fluorimetric procedure for the estimation of free cortisol and corticosterone in 1-ml samples of human plasma has been described. 2. (2) A correction procedure for non-specific fluorescence has been described which is applicable to both human and rat plasma. It depends on the differing rates of development of fluorescence of plasma and of solutions of cortisol and corticosterone. 3. (3) The mean value for free plasma cortisol in 27 normal adults was 9.6 ± 2.7, and for free plasma corticosterone 0.4 ± 0.2 μg/100 ml. Elevated values were foundin Cushing's syndrome, and normal levels in hirsutism and in Addison's disease. Low values occurred in hypopituitarism.

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