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Whole-cell voltage-clamp study of sodium current in neuroblastoma cells: effects of inhibition of neurite outgrowth by colchicine

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
530
Issue
1
Identifiers
DOI: 10.1016/0006-8993(90)90662-u
Keywords
  • Colchicine
  • Neuroblastoma N1E-115
  • Whole-Cell Voltage Clamp
  • Sodium Current
  • Cell Differentiation
  • Cell Culture
Disciplines
  • Biology

Abstract

Abstract A method is described for the production of large numbers of neurite-free neuroblastoma cells that are especially suitable for studies involving whole-cell voltage clamp. Differentiation in the presence of colchicine yielded cells having abundant sodium channels, highly reproducible peak currents and no space-clamp problems. Treatment with this drug did not alter the electrophysiological properties of the cells. Colchicine might be similarly advantageous in voltage-clamp studies of different ion channels and other types of cultured neurons.

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