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Molecular Cloning of a Novel Human Gene (D11S4896E) at Chromosomal Region 11p15.5

Authors
Journal
Genomics
0888-7543
Publisher
Elsevier
Publication Date
Volume
37
Issue
2
Identifiers
DOI: 10.1006/geno.1996.0553
Disciplines
  • Biology
  • Medicine

Abstract

Abstract A novel gene ( GOK) has been cloned from human chromosome region 11p15.5 that is believed to contain a gene or genes associated with a number of pediatric malignancies, including Wilms tumor. A 4-kb cDNA has been cloned and it encodes a predicted protein of ∼84 kDa that could be translated in vitro.Computer analysis predicted that the protein had a signal peptide and may contain a transmembrane helix. Restriction mapping by pulsed-field electrophoresis indicates that GOKis located 1.7 kb telomeric of RRM1,and both genes are transcribed in the same direction. GOKdisplays high evolutionary conservation: cloning and partial sequencing of a mouse genomic clone revealed 90% identity with the human gene at both the nucleotide and the predicted amino acid levels.

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